Strain Data Sheet

RBRC09666

Strain Information

Image
BRC No.RBRC09666
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Gt(ROSA)26Sor<tm2(CAG-cat,-Fam213b)Watk>
Former Common namemPMPGFS KI
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeveloped by Kikuko Watanabe, Koshien University in 2016. RENKA ES cell line (C57BL/6N) was used. Mice were maintained in C57BL/6 background.
Strain descriptionKnock-in mice for Cre-dependent overexpression of mouse Fam213b. Targeted insertion of CAG-loxP-CAT-FRT-PGK-neo-FRT-loxP-Fam213b-pA cassette into the mouse Rosa26 locus.
Colony maintenance
ReferencesJ. Biochem., 150, 593-596 (2011). 21926128
J. Biol. Chem., 283, 792-801 (2008). 18006499
Genes. Dev., 5, 1513-1523 (1991). 1653172

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm2(CAG-cat,-Fam213b)Watk>targeted mutation 2, Kikuko Watanabe

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Fam213bfamily with sequence similarity 213, member B6Fam213b

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
catE. coli chloramphenicol acetyltransferase (CAT)6CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Ordering Information

Donor DNAマウス(Mus musculus) ROSA26, ウシ(Bos taurus) bovine growth hormone polyA signal, サイトメガロウィルス(cytomegalovirus) CMV　promoter, ファージ　P1 (Enterobacteria phage P1) Lox-P, 大腸菌(Escherichia coli) CAT((n) chloramphenicol O-acetyltransferase), SV40(simian virus 40) SV40 polyA, 出芽酵母(Saccharomyces cerevisiae) FRT, マウス(Mus musculus)PGK-1プロモーター, 大腸菌 (Escherichia coli)ネオマイシン耐性遺伝子, マウス(Mus musculus) Fam213b
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biochem., 150, 593-596 (2011). J. Biol. Chem., 283, 492-801 (2008).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the Dr. Kikuko Watanabe is requested.
DepositorKikuko Watanabe (Kansai Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data