Strain Information | |
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Image | |
BRC No. | RBRC09667 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6(CB)-Gt(ROSA)26Sor<tm2.1(CAG-Fam213b)Watk> |
Former Common name | mPMPGFS KI |
H-2 Haplotype | |
ES Cell line | RENKA [C57BL/6NCrlCrlj] |
Background strain | |
Appearance | |
Strain development | Developed by Kikuko Watanabe, Koshien University in 2016. RENKA ES cell line (C57BL/6N) was used. Mice were maintained in C57BL/6 and CBA mixed background. |
Strain description | Knock-in mice for overexpression of mouse Fam213b. Targeted insertion of CAG-loxP-CAT-FRT-PGK-neo-FRT-loxP-Fam213b-pA cassette into the mouse Rosa26 locus. A DNA fragment of loxP-CAT-FRT-PGK-neo-FRT-loxP was removed by crossing to B6;CBA-Tg(CAG-Cre)47Imeg |
Colony maintenance | |
References | J. Biol. Chem., 283, 792-801 (2008). 18006499 Genes. Dev., 5, 1513-1523 (1991). 1653172 J. Biochem., 150, 593-596 (2011). 21926128 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm2.1(CAG-Fam213b)Watk>targeted mutation 2.1, Kikuko Watanabe Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Fam213bfamily with sequence similarity 213, member B6Fam213bCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)6GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP |
Ordering Information | |
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Donor DNA | bovine growth hormone polyA signal, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), phage P1 loxP site, mouse Fam213b cDNA, mouse Gt(ROSA)26Sor genomic DNA |
Research application | Cre/loxP system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biochem., 150, 593-596 (2011). J. Biol. Chem., 283, 492-801 (2008).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the Dr. Kikuko Watanabe is requested. |
Depositor | Kikuko Watanabe (Kansai Medical University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |