Strain Information | |
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Image | |
BRC No. | RBRC09714 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6(C3)-Vsx2<tm1.1(cre/ERT2)Tfur> |
Former Common name | Chx10-IRES-CreERT2 KI |
H-2 Haplotype | |
ES Cell line | JM8A3 [C57BL/6N-A<tm1Brd>] |
Background strain | |
Appearance | |
Strain development | Developed by Takahisa Furukawa, Institute for Protein Research, Osaka University. JM8A3 (C57BL/6N) ES cell line was used. This knock-in mice were mated to mice in B6.C3H background and then mated to mice in C57BL/6J background. |
Strain description | IRES-CreERT2 knock-in mice. An IRES-CreERT2 cassette was placed just downstream of stop codon of the Vsx2 (visual system homeobox 2, Chx10) gene. A Neo gene cassette was removed by a CAG-Cre mouse line. IRES-CreERT2 can be removed by a Flp recombinase. |
Colony maintenance | Heterozygote x Wild-type [or Crossing to C57BL/6JJmsSlc] |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)12ERT2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site12Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site12Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter crePhage P1 Cre recombinase12cre Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP12loxP |
Ordering Information | |
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Donor DNA | yeast FRT (flipase recombination target) sites, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), phage P1 Cre, Human ERT2 (human mutant-type mouse estrogen receptor)cDNA, phage P1 loxP site, mouse Vsx2 genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must perform the study on a collaboration basis with the DEPOSITOR. |
Depositor | Takahisa Furukawa (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |