Strain Data Sheet
RBRC09809
Strain Information | |
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| Image | |
| BRC No. | RBRC09809 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6.Cg-Sharpin<tm1.1Kiwa> |
| Former Common name | SHARPIN conditional KO |
| H-2 Haplotype | |
| ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Kazuhiro Iwai, Graduate School of Medicine and Faculty of Medicine Kyoto University. Bruce4 ES cell line (B6.Cg-Thy1) was used. FRT-flanked Neo gene was removed by crossing a FLP strain. C57BL/6 congenic line. |
| Strain description | Floxed Sharpin(SHANK-associated RH domain interacting protein)mice. Exon 3 to 9 encoding UBL and NZF domains are flanked by loxP sites. |
| Colony maintenance | |
| References | Nat. Commun., 10(1) 3878 (2019). 31462647 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SharpinSHANK-associated RH domain interacting protein15Sharpin<tm1.1Kiwa>targeted mutation 1.1, Kazuhiro Iwai Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site15Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP15loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP15loxP |
Ordering Information | |
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| Donor DNA | Yeast FRT (flippase recombination target) site, Phage P1 loxP sites, mouse Sharpin genomic DNA |
| Research application | Cre/loxP system Dermatology Research FLP/frt system Immunology and Inflammation Research |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Commun., 10(1) 3878 (2019).The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Kazuhiro Iwai (Kyoto University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |