Strain Data Sheet

RBRC09868

Strain Information

Image
BRC No.RBRC09868
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6129-Cbx5<tm1.1Hko>
Former Common nameCbx5 (Hp1 alpha) cond.
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Haruhiko Koseki, RIKEN, in 2010. M1(B6/129) ES cells were used. This strain was backcrossed to C57BL/6J twice.
Strain descriptionFloxed Cbx5 (chromobox 5) mice. A pair of loxP flanks Exon 3.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2022/05/31Room:4-CRack:K

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cbx5chromobox 515Cbx5<tm1.1Hko>targeted mutation 1.1, Haruhiko Koseki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site15Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP15loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP15loxP

Ordering Information

Donor DNAphage P1 loxP sites, yeast FRT (flippase recombination target) site, mouse Cbx5 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsRECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorHaruhiko Koseki (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data