Strain Data Sheet

RBRC09880

Strain Information

Image
BRC No.RBRC09880
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6-Slfn5<em1Tsse>
Former Common nameSLFN5 KO
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Yohei Takeda and Tsukasa Seya, Hokkaido University in 2014. C57BL/6 genetic background.
Strain descriptionSlfn5 (schlafen 5) mutants generated by the CRISPR/Cas9 technique. 1bp insertion was introduced at exon 3.Slfn5<em1Tsse>: 1bp insertion at exon 3.AAGCAAACTCAAACAAGCAGTTCCCTGTGGccatcccacagacttagcctctttcgtgaagctggcctcaagctatgtgggataaagggtcaacttcagaaaggagttcttaaaacttcatttcccacctgctccttcttgttctagtctaatggttctatttttctgggtttcaggaaaagatttcagctaaaggcggagaagatgagtttcctggaggatttggaactgaactttgcggaatgtatacaagatggaggaaaagcgacccttggggttaggcaaagggaggaaatggataccacacactgcatgaaacagaacgaaatcatctcgcaagccgtgtgcgctctgctgaattc(T)cggtggcggagtggtcagggttgagattgagaacggagactataattttgagagggatggagttggcttgaatctgccaccattgttcagaaaccatttagacgagatgctgtatggaaagctctttttaatatatgtgagttcgtgggacgtggcagcctctcacgtgaggctcgctaccctgtgctccaatttgtaccacagatgtGGAACATTTACTGAGGTCATGGATCCTGAA
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Slfn5schlafen 511Slfn5<em1Tsse>endonuclease-mediated mutation 1, Tsukasa Seya

Ordering Information

Donor DNApX330 vector (Streptococcus pyogenes Cas9*, Escherichia coli ampicillin resistant gene, synthetic FLAG tag)* Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested.
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorTsukasa Seya (Hokkaido University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data