Strain Information | |
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Image | |
BRC No. | RBRC09880 |
Type | CRISPR/Cas9 |
Species | Mus musculus |
Strain name | C57BL/6-Slfn5<em1Tsse> |
Former Common name | SLFN5 KO |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Yohei Takeda and Tsukasa Seya, Hokkaido University in 2014. C57BL/6 genetic background. |
Strain description | Slfn5 (schlafen 5) mutants generated by the CRISPR/Cas9 technique. 1bp insertion was introduced at exon 3.Slfn5<em1Tsse>: 1bp insertion at exon 3.AAGCAAACTCAAACAAGCAGTTCCCTGTGGccatcccacagacttagcctctttcgtgaagctggcctcaagctatgtgggataaagggtcaacttcagaaaggagttcttaaaacttcatttcccacctgctccttcttgttctagtctaatggttctatttttctgggtttcaggaaaagatttcagctaaaggcggagaagatgagtttcctggaggatttggaactgaactttgcggaatgtatacaagatggaggaaaagcgacccttggggttaggcaaagggaggaaatggataccacacactgcatgaaacagaacgaaatcatctcgcaagccgtgtgcgctctgctgaattc(T)cggtggcggagtggtcagggttgagattgagaacggagactataattttgagagggatggagttggcttgaatctgccaccattgttcagaaaccatttagacgagatgctgtatggaaagctctttttaatatatgtgagttcgtgggacgtggcagcctctcacgtgaggctcgctaccctgtgctccaatttgtaccacagatgtGGAACATTTACTGAGGTCATGGATCCTGAA |
Colony maintenance | |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter |
Ordering Information | |
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Donor DNA | pX330 vector (Streptococcus pyogenes Cas9*, Escherichia coli ampicillin resistant gene, synthetic FLAG tag)* Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested. |
Research application | |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. |
Depositor | Tsukasa Seya (Hokkaido University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |