Strain Information | |
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Image | |
BRC No. | RBRC10084 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Zfyve27<tm1Kei> |
Former Common name | Prt KO |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Deposited by Keiichi Nakayama, Medical Institute of Bioregulation, Kyusyu University in 2017. E14(129P2/OlaHsd) ES cell line was used. C57BL/6 congenic strain by backcrossing for 7 generations. |
Strain description | Zfyve27 (zinc finger, FYVE domain containing 27, Protrudin) knockout mouse. Mutation in Zfyve27 (Protrudin) has been found in patients of the hereditary spastic paraplegia. This mutant mouse exhibits neurological symptoms resembling mental illness. Exon 1 and 2 were replaced with the Neo gene. |
Colony maintenance | |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Zfyve27zinc finger, FYVE domain containing 2719Zfyve27<tm1Kei>targeted mutation 1, Keiichi I Nakayama Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP19loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP19loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)19mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 PolyA19 |
Ordering Information | |
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Donor DNA | Bacteriophage P1 LoxP sites, mouse phosphoglycerate kinase promoter, E. coli Neomycin resistant gene, SV40 PolyA signal, mouse Zfyve27 genomic DNA |
Research application | Cre/loxP system |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. Contact info: Dr. Michiko Shirane, Ph.D., Nagoya City University <shiram@phar.nagoya-cu.ac.jp> |
Depositor | Keiichi Nakayama (Kyushu University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |