Strain Information | |
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Image | |
BRC No. | RBRC10147 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6-Tg(Actb-PDHL/roGFP1)237Awolf |
Former Common name | Mito-roGFP mice(MRO237) |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Alexnder M Wolf, G Nippon Medical School in 2012. Transgenic construct was injected into fertilized eggs derived from C57BL/6J. Mice were backcrossed to C57BL/6J for more than 10 generations. Mice were maintained as heterozygote. |
Strain description | Transgenic mice expressing ratiometric redox-sensitive GFP (PDHL/roGFP1) in the mitochondria of several tissue under the control of chicken beta-actin promoter. Redox states can be monitored at liver and other tissues. |
Colony maintenance | |
References | J. Invest. Dermatol., 134(6), 1701-1709 (2014). 24129062 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBB2rabbit beta-globin polyAUNHBB2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter PDHA1human Pyruvate dehydrogenase E1 subunit leader sequence (PHDL)UNPDHA1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter roGFP1redox-sensing Green Fluorescent Protein (Aequorea victoria)UNroGFP1chicken beta-actin promoter |
Ordering Information | |
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Donor DNA | Chicken beta-Actin promoter, Human Pyruvate dehydrogenase E1 subunit leader sequence (PHDL) cDNA, Aquorea Victoria GFP variant (C48S/Q80R/S147C/Q204C) cDNA, Rabbit beta-globin PolyA signal |
Research application | Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Alexander M. Wolf (Nippon Medical School) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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No Data |