Strain Information | |
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Image | |
BRC No. | RBRC10385 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129-Pld1<tm1.1Mafr> |
Former Common name | PLD1-KO |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | |
Appearance | |
Strain development | Developed by Yasunori Kanaho and Tsunaki Hongu, University of Tsukuba in 2006. R1 ES cells derived from (129X1/SvJ x 129S1/Sv)F1-Kitl<+> was used, and then mice were backcrossed to C57BL/6J over 10 generations. |
Strain description | Pld1 (phospholipase D1) knockout mice. A loxP site was inserted upstream of Exon 13. An FRT-flanked neo fragment with a 3' loxP site was inserted downstream of exon 13. The neo fragment and Exon 13 were removed by crossing with a Ayu1-Cre mouse. |
Colony maintenance | |
References | Sci. Signal., 5(249) ra79 (2012). 23131846 Mol. Cell Biol., 33(1) 136-145 (2013). 23109426 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP3loxP |
Ordering Information | |
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Donor DNA | phage P1 loxP site, mouse Pld1 genomic DNA |
Research application | Cre/loxP system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Signal., 5(249) ra79 (2012). Mol. Cell Biol., 33(1) 136-145 (2013).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. |
Depositor | Yasunori Kanaho (University of Tsukuba) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |