Strain Information | |
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Image | |
BRC No. | RBRC10939 |
Type | CRISPR/Cas9 |
Species | Mus musculus |
Strain name | C57BL/6N-Slc38a4<em2Smtb> |
Former Common name | Slc38a4 KO |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed and deposited by Shogo Matoba and Atsuo Ogura at RIKEN BRC in 2019. This strain was generated by the CRISPR/Cas9 using fertilized eggs derived from C57BL/6N. |
Strain description | Slc38a4 mutant strain (line B). |
Colony maintenance | |
References | Proc. Natl. Acad. Sci. USA, 116(42):21047-21053 (2019). 31570606 |
Health Report | |
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Examination Date / Room / Rack | 2022/04/25Room:3-CRack:J |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Slc38a4solute carrier family 38, member 415Slc38a4<em2Smtb>endonuclease-mediated mutation 2, Shogo Matoba |
Ordering Information | |
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Donor DNA | Streptococcus pyogenes Cas9* nuclease (human codon optimized), Streptococcus pyogenes tracrRNA(in the form of sgRNA) * Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested. |
Research application | |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, doi: 10.1073/pnas.1907884116.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Atsuo Ogura (RIKEN) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering:
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BRC mice in Publications |
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No Data |