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AES0126  :  Nanog^(βgeo/Hyg)        ( Former No. RCB2697 )
Comment A subline of mouse embryonic stem cell (RF8 derived from 129 SV Jae strain mouse) lacking Nanog expression. Nanog genes were replaced with βgeo gene in one allele and hygromycin resistant gene in another allele. Feeder-free cell line.
Animal mouse
Age embryo
Tissue embryonic stem cells
Exogene βgeo gene, hygr gene
Morphology ES-like
Anchoraged Yes
Medium DMEM(high glucose, Sodium Pyruvate(-))+15%FBS+0.1mM NEAA+0.1mM 2-Mercaptoethanol+1000U/ml mouse LIF
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin+0.02% EDTA, 0.1% gelatin coated dish
Split ratio 2x10^6 cells/100mm dish
Subculture frequency once/2-3days
Cloned No
Lifespan infinite
Mycoplasma -
Isozyme analysis LD, NP
memo feeder : free
Originator Yamanaka, Shinya
Restriction Attach TERMS and CONDITIONS to MTA (C-0034). f
Photo 2014-06-26
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.

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