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AES0192  :  NAT1(+/-)ES(C1)
Comment Mouse embryonic stem (ES) cell. NAT1 gene is deficient heterogeneously.
Animal mouse, 129 SV Jae
Sex Unknown
Age embryo
Tissue embryonic stem cells
Exogene pRTV-NAT1, Neomycin gene
Morphology ES-like
Anchoraged Yes
Medium DMEM(high glucose, Sodium Pyruvate(-))+15%FBS+0.1mM NEAA+0.11mM 2-Mercaptoethanol+1000U/ml mouse LIF
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin+0.02% EDTA, 0.1% gelatin coated dish
Split ratio 1-1.5x10^(6) cells/100mm dish
Subculture frequency once/2-3days
Cloned No
Lifespan infinite
Mycoplasma -
Isozyme analysis animal PCR (isozyme : not tested)
Originator Yamanaka, Shinya
Restriction Attach TERMS and CONDITIONS to MTA (C-0034). f
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.

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RIKEN BioResource Research Center