Please use a Javascript enable browser to see page contents.
CELL SEARCH SYSTEM

APS0003  :  iPS-MEF-Fb/Ng-440A-3
Comment Mouse iPS cell line established without retrovirus vector. It has no integration of exogene. Order Form ( C-0033, C-00034). Please see CiRA , Kyoto University if you are a researcher at a for–profit organization.
Animal mouse
Sex Female
Age embryo
Tissue embryonic fibroblast
Exogene Oct3/4, Sox2, Klf4, c-Myc, Fbx15βgeo/βgeo, Nanog-GFP-IRES-Puro
Morphology ES-like
Anchoraged Yes
Medium DMEM(high glucose, without Sodium Pyruvate)+15%FBS+0.1mM NEAA+0.1mM 2-Mercaptoethanol+1000U/ml mouse LIF
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin+0.02% EDTA, 0.1% gelatin coated dish/SNL76/7
Split ratio 2x10^(6) cells/100mm dish
Subculture frequency SC : once/2-3days, MC : every day
Cloned Yes
Lifespan infinite
Mycoplasma -
Isozyme analysis LD, NP
memo feeder : SNL 76/7 (X-rays:5000R or MMC) 1-2x10^(6) cells/100mm dish
Originator Yamanaka, Shinya
Depositor http://www.cira.kyoto-u.ac.jp/
Restriction 1) The RECIPIENT should read ' the GFP Transfer License contracted between the RIKEN BRC and GE Healthcare Bio-Sciences.' 2) Attach TERMS and CONDITIONS to MTA (C-0034). GFP,f
Photo 2014-05-08
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank@brc.riken.jp) regarding any kind of for-profit use.


back back
RIKEN BioResource Research Center