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CELL SEARCH SYSTEM

APS0007  :  iPS-Hep-FB/Ng/gfp-103C-1
Comment Mouse iPS cell line derived from liver cells. Expressing GFP by CAG promoter. βgeo is knocked-in at Fbx15 locus. GFP and puromycin resistant gene can be expressed by Nanog promoter. Order Form ( C-0033, C-00034). Please see CiRA , Kyoto University if you are a researcher at a for–profit organization.
Animal mouse
Sex Male
Age 21 weeks
Tissue liver
Exogene pMXs, Oct3/4, Sox2, Klf4, c-Myc, GFP, βgeo gene
Morphology ES-like
Anchoraged Yes
Medium DMEM(high glucose, without Sodium Pyruvate)+15%FBS+0.1mM NEAA+0.1mM 2-Mercaptoethanol+1000U/ml mouse LIF
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin+0.02% EDTA, 0.1% gelatin coated dish/SNL76/7
Split ratio 2x10^(6) cells/100mm dish
Subculture frequency SC : once/2-3days, MC : every day
Cloned Yes
Lifespan infinite
Mycoplasma -
Isozyme analysis LD, NP
memo feeder : SNL76/7 (X-rays:5000R or MMC) 1-2x10^(6) cells/100mm dish
Originator Yamanaka, Shinya
Depositor http://www.cira.kyoto-u.ac.jp/
Restriction 1) The RECIPIENT should read ' the GFP Transfer License contracted between the RIKEN BRC and GE Healthcare Bio-Sciences.' 2) Attach TERMS and CONDITIONS to MTA (C-0034). GFP,f
Photo 2014-05-08
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank@brc.riken.jp) regarding any kind of for-profit use.


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