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HPS0294  :  CiRA00026
Comment Disease specific iPS cell line derived from a patient : Amyotrophic lateral sclerosis. HPS0292 and HPS0293 were derived from the same patient. Order Form (C-0042, C-0057, C-0007).
Animal human
Subspecies Japanese
Tissue skin
Exogene Oct3/4, Sox2, Klf4, L-Myc, Lin28, shp53
Morphology ES-like
Anchoraged Yes
Medium Primate ES Cell Medium+4ng/ml human basic FGF
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin+0.1% collagenase type IV+20% KSR+1mM CaCl2+PBS(-), 0.1% gelatin coated dish
Split ratio 1:3-5 split
Subculture frequency subculture : once/4-6days. medium change : every day
Lifespan infinite
Mycoplasma -
Short Tandem Repeat OK
memo DAP213 : Medium+2M DMSO+1M Acetamide+3M Propyleneglycol, feeder : SNL 76/7 (X-rays:5000R or MMC) 1.5-2.5x10^(6) cells/100mm dish
Restriction 1) The BIOLOGICAL RESOURCE must be used for academic research purpose conducted by RECIPIENT who belongs to a not-for profit academic organization (i.e. a university or another institution of higher education or any non-profit scientific or educational organization, including government agencies). 2) The RECIPIENT agrees to obtain the prior written approval of the distribution from the APPROVER. 3) Contact Us : b
Photo 2014-05-20
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.

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