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HPS0360  :  648A1
Comment Human iPS cell line derived from a healthy individual (peripheral blood)l. Established with episomal vector and no exogenous gene is present in the cell line. Oct3/4, Sox2, Klf4, L-Myc, Lin28 and shRNA against p53 were used for establishment. Order Form ( C-0042, C-0034).
Animal human
Sex Male
Age 30s
Tissue peripheral blood
Exogene Oct3/4, Sox2, Klf4, L-Myc, Lin28, shp53
Morphology ES-like
Anchoraged Yes
Medium Primate ES Cell Medium+4ng/ml human basic FGF
Antibiotics Free
Growth temp 37oC
CO2 concentration 5%
Passage method 0.25% trypsin+0.1% collagenase type IV+20% KSR+1mM CaCl2+PBS(-), 0.1% gelatin coated dish
Split ratio 1:3-6 split
Subculture frequency subculture : once/4-6days. medium change : every day
Lifespan infinite
Mycoplasma -
Virus HTLV-1:(-), HIV:undetected
Chr distrb 46XY(20)
Short Tandem Repeat OK
memo DAP213 : Medium+2M DMSO+1M Acetamide+3M Propyleneglycol, feeder : SNL 76/7 (X-rays:5000R or MMC) 1.5-2.5x10^(6) cells/100mm dish
Depositor Yamanaka, Shinya
Restriction Attach TERMS and CONDITIONS to MTA (C-0034). f
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.

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